| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 131 | Megasphaera elsdenii | TCGTCGTCGGCAATGTGA | CGTGCCAATGCTTGACGT | 59.35 | 59.05 | 192 | 74 |
100.00%
|
100.00%
|
| Primer 132 | Megasphaera elsdenii | CATGCACCGCAAACTGGT | TTTGCGGTTCGGTTCCCA | 58.96 | 59.81 | 163 | 74 |
100.00%
|
100.00%
|
| Primer 133 | Megasphaera elsdenii | TCCGGAAGACAACCAGCAC | TGCTGGGTTCAAAGCGCT | 59.93 | 60.20 | 293 | 74 |
100.00%
|
100.00%
|
| Primer 134 | Megasphaera elsdenii | TGGCCTGAAGCTGGACATG | TTTCCCAGCCTTGCGGAA | 60.00 | 59.48 | 160 | 74 |
100.00%
|
100.00%
|
| Primer 135 | Megasphaera elsdenii | ACGGCACAAATCGTCGCT | TTCCCAGGGTTTCGACGTG | 60.36 | 59.93 | 256 | 74 |
100.00%
|
100.00%
|
| Primer 136 | Megasphaera elsdenii | ACGGCACAAATCGTCGCT | TCCAAGGTCCCCAGGATGT | 60.36 | 59.84 | 224 | 74 |
100.00%
|
100.00%
|
| Primer 137 | Megasphaera elsdenii | ACGGCACAAATCGTCGCT | AGGGTTTCGACGTGATCCG | 60.36 | 59.79 | 251 | 74 |
100.00%
|
100.00%
|
| Primer 138 | Megasphaera elsdenii | ACGGCACAAATCGTCGCT | GCCTCATCTCCGCTGAACA | 60.36 | 59.78 | 176 | 74 |
100.00%
|
100.00%
|
| Primer 139 | Megasphaera elsdenii | ACGGCACAAATCGTCGCT | CCCAGGGTTTCGACGTGAT | 60.36 | 59.70 | 254 | 74 |
100.00%
|
100.00%
|
| Primer 140 | Megasphaera elsdenii | AGAGCGCTGTCTTCAACGT | ATCCGTGGCAAAGGCGAT | 59.64 | 59.73 | 224 | 74 |
100.00%
|
100.00%
|